Job Description

Research Technician

This is a terrific opportunity within the Paulovich lab for a highly motivated and upper level full-time Research Technician to join our translational proteomics team to help drive translational projects forward towards clinical applications by performing bench-level experiments in support of proteomic, genomic, and transcriptomic analyses of clinical and preclinical biospecimens. The ideal candidate will be able to work as part of a team as well as independently to follow established standard operating protocols (SOP) in a CLIA/CAP laboratory environment.

Responsibilities

1. Process proteomic samples for mass spectrometry analysis (e.g., protein extraction, fractionation, immuno-depletion, trypsin digestion, alkylation, reduction, etc.)
2. Assist in generation and analysis of mass spectrometry-based proteomic data
3. Follow standard operating protocols (SOP) in a CLIA/CAP laboratory environment including participating in annual competency and proficiency testing
4. Work with antibodies (antibody crosslinking/purification, immunoaffinity purification, immobilization on solid supports, ELISAs)
5. Collaborate effectively with an interdisciplinary team spanning expertise in medicine, biology, analytical chemistry, and statistics
6. Design and present Powerpoint presentations of results to colleagues and collaborators
7. Maintain laboratory equipment, records, research results files, and databases
8. Write progress reports and develop standard operating protocols (SOPs)
9. Write and co-author scientific manuscripts (in peer-reviewed journals)
10. Perform other duties as required

Qualifications

MINIMUM QUALIFICATIONS:

• Bachelor's degree in related scientific field or equivalent combination of education and experience
• 8 years of related work experience (or equivalent combination of advanced degree and work experience)
• Proven ability and knowledge necessary to conduct research independently

PREFERRED QUALIFICATIONS:

• Master's degree or higher in Protein Chemistry, Analytical Chemistry, Clinical Chemistry, Biochemistry, Molecular Biology or related discipline. Salary and title based on experience.
• Previous experience in a CLIA/CAP or other regulated environment
• Previous experience with Tandem Mass Tag (TMT) labeling, data-dependent acquisition (DDA) and data-independent acquisition (DIA) mass spectrometry techniques
• Previous experience with multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) targeted mass spectrometry techniques
• Previous experience isolating human peripheral blood mononuclear cells (PBMC) for proteomics experiments- isolation from whole blood, expansion in culture, perturbation with chemical agents, protein extraction and quantification, etc.
• Previous experience with QC and processing of formalin-fixed paraffin-embedded (FFPE) and flash-frozen specimen processing and protein extraction for proteomics experiments
• Previous experience in mass spectrometry data analysis tools (such as Proteome Discoverer, Fragpipe, Skyline, etc.)
• Previous experience with quantitative image analysis for pathology (such as Indicta Labs HALO software)
• Previous work with antibodies and peptide standards (immunoaffinity purification, immobilization on solid supports, preparation of samples for mass spectrometry)
• Previous experience with liquid handling robot usage and programming (Python, R)
• Ability to perform traditional biochemistry experiments on human and animal biospecimens and cell lines
• Previous work maintaining and running mass spectrometry and HPLC instruments to generate proteomic data
• Strong work ethic and ability to multi-task efficiently in a highly interactive, interdisciplinary team environment
• Comfort in a milestone-driven environment, with quarterly objectives
• Exquisite attention to detail and the ability to adhere to standard operating protocols (SOP) and maintain records in a CLIA-Cap environment.
• Proficiency with MS Office programs (Word, Powerpoint, Excel, and Access)
• Willingness to learn new techniques
• Excellent English writing and oral communication skills.
• A strong publication record

The annual base salary range for this position is from $80,475 to $120,681, and pay offered will be based on experience and qualifications.

This position may be eligible for relocation assistance. This position is not eligible for H-1B sponsorship at this time.

Fred Hutchinson Cancer Center offers employees a comprehensive benefits package designed to enhance health, well-being, and financial security. Benefits include medical/vision, dental, flexible spending accounts, life, disability, retirement, family life support, employee assistance program, onsite health clinic, tuition reimbursement, paid vacation (12-22 days per year), paid sick leave (12-25 days per year), paid holidays (13 days per year), and paid parental leave (up to 4 weeks).

We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to Human Resources at hrops@fredhutch.org or by calling 206-667-4700.

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